July 2012
Volume 53, Issue 8
Free
Letters to the Editor  |   July 2012
An Analysis of the Use of Multiple Comparison Corrections in Ophthalmology Research
Author Affiliations & Notes
  • Carrie Huisingh
    Center for Clinical and Translational Sciences, University of Alabama at Birmingham, Birmingham, Alabama; and Departments of
  • Gerald McGwin, Jr
    Epidemiology and
  • Corresponding author: Gerald McGwin, Departments of Epidemiology and Ophthalmology, University of Alabama at Birmingham, 609 Callahan Eye Hospital, 1720 University Boulevard, Birmingham, AL 35294; mcgwin@uab.edu
Investigative Ophthalmology & Visual Science July 2012, Vol.53, 4777. doi:10.1167/iovs.12-10336
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Carrie Huisingh, Gerald McGwin; An Analysis of the Use of Multiple Comparison Corrections in Ophthalmology Research. Invest. Ophthalmol. Vis. Sci. 2012;53(8):4777. doi: 10.1167/iovs.12-10336.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Introduction
Weread with great interest the article, “An analysis of the use of multiple comparison corrections in ophthalmology research.” 1 The authors reviewed abstracts from the 2012 ARVO Meeting to estimate the number of abstracts reporting false-positive results or where multiple comparison corrections needed to be considered. We would like to comment on the authors' methodology as well as provide a counterpoint to their presentation of the multiple comparison corrections. 
Several limitations to the methodology are worth noting. First, the words “significant” and “statistically significant” were not included in the list of search terms. It is quite possible that an abstract makes reference to statistically significant results, but is devoid of P values, and presumably would not have been included in their search (Gallagher MJ, et al. IOVS 2010;51:ARVO E-Abstract 4555). Second, it also is quite possible that an abstract may not contain any mention of a multiple comparisons correction, but the actual paper does (Bousquet E, et al. IOVS 2011;52:ARVO E-Abstract 6582; Lavery W, et al. IOVS 2010; 51:ARVO E-Abstract 5036; Millard LH, et al. IOVS 2010;51:ARVO E-Abstract 151). 24 Given space constraints in a conference abstract, it would not be unusual for this information to be omitted. Thus, the authors likely have underestimated the number of analyses that corrected for multiple comparisons. Furthermore, they define abstracts reporting five or more P values as those needing the use of a correction factor; however, they provide no scientific explanation for this criterion, nor do the authors use this criterion consistently in their own scientific research. 5,6  
The theoretical basis behind adjusting for multiple comparisons is relatively straightforward. However, as noted above, we wish to provide some practical counterpoints to what the authors infer is a straightforward and universally accepted process. 
First and foremost, the process of enumerating the relevant number of statistical tests is vital and has implications for the proper interpretation of the corrected results. 79 However, there are a number of important and often unanswered questions in this regard. What about tests that were performed, but not published? What about tests planned for future studies? What about other tests the investigator has done in her or his lifetime? This lack of consistency creates some practical problems. For example, imagine two published studies on the same topic, both adjusted for multiple comparisons. In theory, if the method of adjustment (e.g., Bonferroni, Tukey, Newman-Keuls), critical value used as the acceptable alpha level, or the number of other tests performed were different, the same results would be interpreted differently between the two studies. This defies common sense. As such, the lack of a gold standard approach for multiple test adjustment makes comparing results from one study to another very difficult. 
The authors suggest that the paucity of multiple comparison corrections may produce unwarranted shifts in clinical or surgical care. Adjusting for multiple comparisons can cut both ways, as it results in a higher type II error rate by artificially setting the bar for statistical significance very high. 8,10 While it is more conservative than the conventional P < 0.05, applying adjustment factors hampers researchers from taking on a true association and exploring it further. 8,10 In terms of clinical care, this means standard practices could continue being used when, in fact, a shift is warranted. In addition, the authors provide no evidence that failure to adjust has led to any shift in clinical care. In fact, common sense dictates that any changes in clinical care will not be based on the results of a single paper, corrected for multiple comparisons or not; changes in clinical care happen in an evolutionary manner. It is our belief that the lack of a standardized approach for multiple test adjustment is more likely to hamper shifts in clinical care than concern for the number of false-positive results, as the author suggests. 
The recommended approach when dealing with multiple tests is to make use of the effect size in addition to the P value. In addition, authors should point out any methodologic concerns that are relevant for making a quality explanation of an association, such as control selection, nonresponse, recall bias related to disease occurrence, interviewer bias, confounding, and prior evidence. 8 This should enable the reader to reach a reasonable conclusion without the help of adjustments. 
References
Stacey AW Pouly S Czyz CN. An analysis of the use of multiple comparison corrections in ophthalmology research. Invest Ophthalmol Vis Sci . 2012; 53:1830–1834. [CrossRef] [PubMed]
Bousquet E Camelo S les Jardins GL Protective effect of intravitreal administration of tresperimus, an immunosuppressive drug, on experimental autoimmune uveoretinitis. Invest Ophthalmol Vis Sci . 2011; 52:5414–5423. [CrossRef] [PubMed]
Lavery W Muir E Kiel J Duong T. Magnetic resonance imaging indicates decreased choroidal and retinal blood flow in the DBA/2J mouse model of glaucoma. Invest Ophthalmol Vis Sci . 2012; 53:560–564. [CrossRef] [PubMed]
Millard L Woodward D Stamer W. The role of the prostaglandin EP4 receptor in the regulation of human outflow facility. Invest Ophthalmol Vis Sci . 2011; 52:3506–3513. [CrossRef] [PubMed]
Czyz CN Kalwerisky K Stacey AW Initial treatment of ocular exposure and associated complications in severe periorbital thermal injuries. J Trauma . 2011; 71:1455–1459. [CrossRef] [PubMed]
Czyz CN Foster JA Lam VB Efficacy of pulsed electromagnetic energy in postoperative recovery from blepharoplasty. Dermatol Surg . 2012; 38:445–450. [CrossRef] [PubMed]
Thomas D Siemiatycki J Dewar R Robins J Goldberg M Armstrong B. The problem of multiple inference in studies designed to generate hypothesis. Amer J Epidemiol . 1985; 122:1080–1095.
Savitz DA Olshan AF. Multiple comparisons and related issues in the interpretation of epidemiologic data. Amer J Epidemiol . 1995; 142:904–908.
Thompson JR. Invited commentary: Re: “Multiple comparisons and related issues in the interpretation of epidemiologic data.” Amer J Epidemiol . 1998; 147:801–806. [CrossRef]
Rothman KJ. No adjustments are needed for multiple comparisons. Epidemiology . 1990;1:43–46. [CrossRef] [PubMed]
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×