October 1984
Volume 25, Issue 10
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Articles  |   October 1984
The growth of endothelium from human corneal rims in tissue culture.
Investigative Ophthalmology & Visual Science October 1984, Vol.25, 1213-1216. doi:
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      S K Nayak, P S Binder; The growth of endothelium from human corneal rims in tissue culture.. Invest. Ophthalmol. Vis. Sci. 1984;25(10):1213-1216.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The authors report the successful in vitro growth of human corneal endothelium derived from 12 of 31 corneal-scleral rims obtained following corneal transplantation. The average age of donors whose endothelium grew was 17.6 +/- 3.2 years. The average interval between death and culturing was 51.5 +/- 10.1 hr. The cells migrated from the explants within 3-7 days and were characterized by a flattened, polygonal shape with a centrally located nucleus. Cell growth exhibited a doubling time of 72-96 hr in the second and third tissue culture passages. A reduced growth rate was observed by cell lines maintained in vitro for over 6 months. Fibroblast growth factor, epidermal growth factor, and endothelial cell growth supplement all exerted a positive influence on cell proliferation. Corneal-scleral rims can be a valuable source of endothelial cells for corneal research.

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