August 1984
Volume 25, Issue 8
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Articles  |   August 1984
Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining.
Investigative Ophthalmology & Visual Science August 1984, Vol.25, 977-980. doi:
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      J S Zigler, M Mochizuki, T Kuwabara, I Gery; Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining.. Invest. Ophthalmol. Vis. Sci. 1984;25(8):977-980.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obtain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 column. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.

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