November 1988
Volume 29, Issue 11
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Articles  |   November 1988
Identification of proteins in retinas and IPM from eyes with retinitis pigmentosa.
Author Affiliations
  • S Y Schmidt
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
  • C A Heth
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
  • R B Edwards
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
  • J T Brandt
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
  • A J Adler
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
  • A Spiegel
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
  • H Shichi
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
  • E L Berson
    Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston 02114.
Investigative Ophthalmology & Visual Science November 1988, Vol.29, 1585-1593. doi:
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      S Y Schmidt, C A Heth, R B Edwards, J T Brandt, A J Adler, A Spiegel, H Shichi, E L Berson; Identification of proteins in retinas and IPM from eyes with retinitis pigmentosa.. Invest. Ophthalmol. Vis. Sci. 1988;29(11):1585-1593.

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Abstract

Opsin, the alpha-subunit of transducin, S-antigen, interphotoreceptor retinoid-binding protein (IRBP) and cathepsin D were assessed in autopsy eyes from patients with retinitis pigmentosa (RP) and normal autopsy eyes. Immunochemical methods were used to determine the presence of these proteins on Western blots of retinal homogenates from five RP donors and on blots of interphotoreceptor matrix (IPM) preparations from six other RP eyes. The amounts of immunoreactive opsin, S-antigen, alpha-transducin, and IRBP appeared below normal in retinas from RP eyes. All six IPM samples from patients with advanced RP had reduced amounts of S-antigen and no detectable IRBP or transducin. Cathepsin D (an RPE protein) was present in IPM or RP eyes in amounts comparable to that in IPMs from normal eyes. Small amounts of cathepsin D were also detected in retinas from both normal and RP eyes. These studies show that proteins specific to the photoreceptor-pigment epithelium complex in normal eyes can be detected in autopsy eyes from patients with RP and suggest that the observed reductions in photoreceptor-specific proteins occur as a consequence of photoreceptor loss.

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