August 1988
Volume 29, Issue 8
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Articles  |   August 1988
A scanning ocular spectrofluorophotometer.
Author Affiliations
  • J W McLaren
    Department of Ophthalmology, Mayo Clinic, Rochester, MN 55905.
  • R F Brubaker
    Department of Ophthalmology, Mayo Clinic, Rochester, MN 55905.
Investigative Ophthalmology & Visual Science August 1988, Vol.29, 1285-1293. doi:
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      J W McLaren, R F Brubaker; A scanning ocular spectrofluorophotometer.. Invest. Ophthalmol. Vis. Sci. 1988;29(8):1285-1293.

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      © 2016 Association for Research in Vision and Ophthalmology.

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Abstract

We describe an instrument called a scanning ocular spectrofluorophotometer (SOSF) that measures fluorescence in a two-dimensional cross-section through the anterior chamber and cornea and provides the ability to change excitation and emission wavelengths rapidly. The output of a xenon arc lamp is filtered by a diffraction grating monochromator which has a bandpass of 4 nm and a range of 400 to 800 nm. Light emitted from the fluorophore is filtered by a variable wavelength interference filter which has a bandpass of approximately 11 nm and a range of 400 to 700 nm. To demonstrate the versatility of the instrument, we measured the spectra of fluorescein, fluorescein glucuronide and rhodamine B in the anterior chambers and corneas of pigmented rabbits after topical administration. We also measured simultaneously and independently the redistribution and disappearance of a mixture of fluorescein-labeled dextran and rhodamine B after intracameral injection. Rhodamine B was very rapidly absorbed by the cornea and lens while fluorescein-dextran was not measurable in the cornea before 4 hr. The SOSF provides a means of carrying out spectrofluorophotometry in the living eye and carrying out kinetic experiments which would otherwise be awkward or impossible.

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