September 1988
Volume 29, Issue 9
Free
Articles  |   September 1988
In vitro immunological function of human corneal fibroblasts.
Author Affiliations
  • E Young
    Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
  • W J Stark
    Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Investigative Ophthalmology & Visual Science September 1988, Vol.29, 1402-1406. doi:
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      E Young, W J Stark; In vitro immunological function of human corneal fibroblasts.. Invest. Ophthalmol. Vis. Sci. 1988;29(9):1402-1406.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Gamma-interferon-induced HLA-DR-like antigens on normal human fibroblasts were compared functionally to HLA-DR antigens on peripheral blood mononuclear leucocytes (PBML) from the same individual. Irradiated DR+ fibroblasts did not stimulate allogeneic PBML to divide in a mixed lymphocyte fibroblast culture (MLFC) as determined by 3H-thymidine incorporation, although lymphocytes autologous to the fibroblasts stimulated normally. Co-culture with interleukin 1 (IL-1), IL-2, 2-mercaptoethanol and indomethacin had no effect on MLFC. Pretreatment of stimulator fibroblasts with neuraminidase to modify sialoglycoproteins increased responses only modestly. Both DR+ and DR- fibroblasts inhibited a normal mixed lymphocyte culture (MLC) response in a dose-dependent fashion. These results suggest that the induction of HLA-DR-like molecules on fibroblasts is not sufficient to initiate host sensitization and that fibroblasts may inhibit normal lymphoproliferation.

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