January 1989
Volume 30, Issue 1
Free
Articles  |   January 1989
Characterization of beta-adrenergic receptors in cultured human trabecular cells and in human trabecular meshwork.
Author Affiliations
  • M B Wax
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
  • P B Molinoff
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
  • J Alvarado
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
  • J Polansky
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
Investigative Ophthalmology & Visual Science January 1989, Vol.30, 51-57. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      M B Wax, P B Molinoff, J Alvarado, J Polansky; Characterization of beta-adrenergic receptors in cultured human trabecular cells and in human trabecular meshwork.. Invest. Ophthalmol. Vis. Sci. 1989;30(1):51-57.

      Download citation file:


      © 2017 Association for Research in Vision and Ophthalmology.

      ×
  • Supplements
Abstract

The characterization of beta-adrenergic receptors on cultured human trabecular cells and trabecular meshwork from human autopsy eyes was carried out by radioligand binding utilizing (125I)-iodopindolol. In cultured cells, the observed binding of (125I)-iodopindolol was of high affinity (Kd = 43 pM) and saturable. Scatchard plots were linear and revealed a Bmax of 33 +/- 7 fmol/mg of protein. Competition studies with a series of agonists and antagonists revealed that human trabecular cells contain a single class of beta-adrenergic receptors of the beta 2 subtype. Similarly, the IC50 of ICI 89,406 (176 nM) in human trabecular meshwork from autopsy eyes supports the presence of beta 2-adrenergic receptors in this tissue.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×