October 1991
Volume 32, Issue 11
Free
Articles  |   October 1991
An inhibitor of the matrix metalloproteinase synthesized by rabbit corneal epithelium.
Author Affiliations
  • M E Fini
    Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
  • T Y Cui
    Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
  • A Mouldovan
    Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
  • D Grobelny
    Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
  • R E Galardy
    Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
  • S J Fisher
    Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Investigative Ophthalmology & Visual Science October 1991, Vol.32, 2997-3001. doi:
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    • Get Citation

      M E Fini, T Y Cui, A Mouldovan, D Grobelny, R E Galardy, S J Fisher; An inhibitor of the matrix metalloproteinase synthesized by rabbit corneal epithelium.. Invest. Ophthalmol. Vis. Sci. 1991;32(11):2997-3001.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Normal and abnormal processes of cellular invasion often are initiated by degradation of basement membranes. The process of corneal ulceration might operate via similar mechanisms; degradation of the corneal stroma is not seen until after the basement membrane underlying the corneal epithelium in the preulcerative lesion is lost. Recent data implicate a member of the matrix metalloproteinase (MMP) family of enzymes, 92 kD gelatinase/type IV collagenase (MMP-9) in both cellular invasion processes and degradation of epithelial basement membrane before corneal ulceration. This suggests that use of nontoxic substances that block activity of MMP-9 might be useful in preventing or inhibiting pathologic invasion processes in vivo. An agent that fits these criteria is N-[D,L-2-isobutyl-3(N'-hydroxycarbonylamido)-propanoyl]-O- methyl-L-tyrosine methylamide, which previously has been characterized as an inhibitor of tumor cell collagenases. In this study, the authors show that the inhibitor can efficiently block activity of MMP-9 purified from cultures of rabbit corneal epithelial cells. Results suggest that the recently reported efficacy of a closely related inhibitor in blocking progression of alkali burns to ulceration might be attributable to its action against MMP-9.

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