September 1991
Volume 32, Issue 10
Free
Articles  |   September 1991
Effects of oxidized glutathione on ATPase activities in rat retina.
Author Affiliations
  • B S Winkler
    Eye Research Institute, Oakland University, Rochester, Michigan 48309.
  • F J Solomon
    Eye Research Institute, Oakland University, Rochester, Michigan 48309.
  • S M Orselli
    Eye Research Institute, Oakland University, Rochester, Michigan 48309.
Investigative Ophthalmology & Visual Science September 1991, Vol.32, 2840-2842. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      B S Winkler, F J Solomon, S M Orselli; Effects of oxidized glutathione on ATPase activities in rat retina.. Invest. Ophthalmol. Vis. Sci. 1991;32(10):2840-2842.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

The activities of Mg(2+)-dependent and Na(+)-K(+)-stimulated ATPase in homogenates of rat retina were measured in the presence of increasing concentrations of oxidized glutathione (GSSG). The Mg(2+)-ATPase was not inhibited by GSSG at any of the concentrations tested. The Na(+)-K(+)-stimulated ATPase was not inhibited by 1 mM GSSG, but its activity was decreased by 20 and 35%, respectively, in the presence of 5 and 10 mM GSSG. Other enzymatic measurements using supernatant fractions of rat retina showed that 1-10 mM GSSG did not inhibit the activities of hexokinase, glucose-6-phosphate dehydrogenase, or glyceraldehyde-3-phosphate dehydrogenase. These results suggest that GSSG is not likely to exert significant deleterious changes on cellular processes, at least in cells and tissues in which normal glutathione (GSH) concentration is 2 mM or lower.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×