October 1991
Volume 32, Issue 11
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Articles  |   October 1991
Cultured retinal pigment epithelium cells from donors with type I diabetes show an altered insulin response.
Author Affiliations
  • M V Miceli
    LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans.
  • D A Newsome
    LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans.
Investigative Ophthalmology & Visual Science October 1991, Vol.32, 2847-2853. doi:
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      M V Miceli, D A Newsome; Cultured retinal pigment epithelium cells from donors with type I diabetes show an altered insulin response.. Invest. Ophthalmol. Vis. Sci. 1991;32(11):2847-2853.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Retinal pigment epithelium (RPE) isolated from 13 of 49 eyes of donors with insulin-dependent diabetes produced viable, proliferating cultures. No difference was found in baseline glucose uptake and lactate production, as measured by 13C and 1H nuclear magnetic resonance, between the RPE cells from diabetic donors and those cultured from normal donors. Insulin-mediated stimulation of glucose uptake and lactate production was decreased significantly in the RPE cells from diabetic donors compared with those from normal controls. Oxygen consumption and the percentage of endogenous oxygen consumption from fatty-acid oxidation, determined by using a specific inhibitor, were similar in both groups. Cellular proliferation and endocytosis, measured by liposome uptake, also were stimulated by insulin similarly in both types of cells. These results show that at least one or more mechanisms of insulin action in the RPE, thought to be a noninsulin-dependent tissue, may be altered permanently by chronic insulin-dependent diabetes. This finding may have implications for the pathogenesis of disease in noninsulin-dependent tissues even in patients with tight glycemic control.

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