September 1991
Volume 32, Issue 10
Free
Articles  |   September 1991
Prevention of herpes keratitis by monoclonal antibodies specific for discontinuous and continuous epitopes on glycoprotein D.
Author Affiliations
  • R N Lousch
    Department of Microbiology/Immunology, University of South Alabama, Mobile 36688.
  • H Staats
    Department of Microbiology/Immunology, University of South Alabama, Mobile 36688.
  • J E Oakes
    Department of Microbiology/Immunology, University of South Alabama, Mobile 36688.
  • G H Cohen
    Department of Microbiology/Immunology, University of South Alabama, Mobile 36688.
  • R J Eisenberg
    Department of Microbiology/Immunology, University of South Alabama, Mobile 36688.
Investigative Ophthalmology & Visual Science September 1991, Vol.32, 2735-2740. doi:
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    • Get Citation

      R N Lousch, H Staats, J E Oakes, G H Cohen, R J Eisenberg; Prevention of herpes keratitis by monoclonal antibodies specific for discontinuous and continuous epitopes on glycoprotein D.. Invest. Ophthalmol. Vis. Sci. 1991;32(10):2735-2740.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Seven monoclonal antibodies (mAb) specific for defined discontinuous and continuous epitopes on glycoprotein D of herpes simplex virus type 1 (HSV-1) were surveyed for their capacity to protect against virus-induced corneal disease in a murine ocular infection model. A known amount of purified mAb was transferred passively to BALB/c mice 24 hr after topical infection with HSV-1 on their scarified corneas. At high doses (50-136 micrograms), all seven mAbs protected against the development of persistent necrotizing stromal keratitis. Significant protection was also observed at low doses (20 micrograms) with two mAbs to discontinuous epitopes and two mAbs to continuous epitopes. Selected high-dose mAbs also were able to reduce the severity of blepharitis. These results indicated that at least seven different antigenic sites on glycoprotein D can serve as targets for effective antibody therapy in the murine model of HSV-1 ocular infection.

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