January 1991
Volume 32, Issue 1
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Articles  |   January 1991
Neutrophil chemotactic factors in the tears of giant papillary conjunctivitis patients.
Author Affiliations
  • S A Elgebaly
    Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
  • P C Donshik
    Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
  • F Rahhal
    Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
  • W Williams
    Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
Investigative Ophthalmology & Visual Science January 1991, Vol.32, 208-213. doi:
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      S A Elgebaly, P C Donshik, F Rahhal, W Williams; Neutrophil chemotactic factors in the tears of giant papillary conjunctivitis patients.. Invest. Ophthalmol. Vis. Sci. 1991;32(1):208-213.

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Abstract

This study was designed to determine the presence of neutrophil chemotactic factors in the tears of patients with giant papillary conjunctivitis (BPC) secondary to contact lenses. Chemotactic activity was measured using modified Boyden chambers and the chemoattractant formylmethionyl-leucyl-phenylalanine (f-MLP) for 100 percent response. Elevated levels of chemotactic activity were found in the tears of symptomatic patients (80.8 +/- 6.4, % f-MLP) compared with control tears of asymptomatic contact lens wearers (15.7 +/- 3.3%) and non-contact lens wearers (5.6 +/- 1.2%). Using radioimmunoassay, C5a (serum-derived chemoattractant), leukotriene-B4, and interleukin-1 (immune cell-derived chemoattractants) were not detected in the tears of symptomatic patients. The authors determined whether injured conjunctival cells participate in this process by releasing neutrophil chemotactic factors. Isolated rabbit bulbar conjunctiva incubated with culture medium for 4 and 6 hr released high levels of neutrophil chemotactic factors. The release of these factors from injured conjunctiva support the premise that physical trauma of conjunctival cells induced by contact lenses may be an important component of the pathophysiology of giant papillary conjunctivitis.

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