September 1991
Volume 32, Issue 10
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Articles  |   September 1991
Morphologic effects of bacteriochlorin a and light in vivo on intraocular melanoma.
Author Affiliations
  • J J Schuitmaker
    Department of Ophthalmology, Academic Hospital, Leiden, The Netherlands.
  • G F Vrensen
    Department of Ophthalmology, Academic Hospital, Leiden, The Netherlands.
  • J L van Delft
    Department of Ophthalmology, Academic Hospital, Leiden, The Netherlands.
  • D de Wolff-Rouendaal
    Department of Ophthalmology, Academic Hospital, Leiden, The Netherlands.
  • T M Dubbelman
    Department of Ophthalmology, Academic Hospital, Leiden, The Netherlands.
  • A de Wolf
    Department of Ophthalmology, Academic Hospital, Leiden, The Netherlands.
Investigative Ophthalmology & Visual Science September 1991, Vol.32, 2683-2688. doi:
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      J J Schuitmaker, G F Vrensen, J L van Delft, D de Wolff-Rouendaal, T M Dubbelman, A de Wolf; Morphologic effects of bacteriochlorin a and light in vivo on intraocular melanoma.. Invest. Ophthalmol. Vis. Sci. 1991;32(10):2683-2688.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

To study the development of tissue and cell damage, the early morphologic changes induced by photodynamic therapy (PDT) with the new photosensitizer bacteriochlorin a (BCA) were investigated in Greene hamster melanoma implanted in the anterior eye chamber of white rabbits up to 24 hr after BCA-PDT, using light and electron microscopy. Immediately after BCA-PDT, intracellular spaces were enlarged, and blood vessels were clotted with swollen erythrocytes. By electron microscopy, it was found that some mitochondria had fused inner and outer membranes, and the cristae mitochondriales were affected. With time, the severity of the tissue and cell damage increased, leading to almost complete tumor necrosis after 24 hr. The direct mitochondrial damage and the vascular damage induced by BCA-PDT probably both contribute to tumor necrosis.

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