May 1991
Volume 32, Issue 6
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Articles  |   May 1991
Identification of the 2A10 antigen of retinal pigment epithelium as a beta 1 subunit of integrin.
Author Affiliations
  • P Chu
    Department of Anatomy, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107.
  • G B Grunwald
    Department of Anatomy, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107.
Investigative Ophthalmology & Visual Science May 1991, Vol.32, 1757-1762. doi:
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      P Chu, G B Grunwald; Identification of the 2A10 antigen of retinal pigment epithelium as a beta 1 subunit of integrin.. Invest. Ophthalmol. Vis. Sci. 1991;32(6):1757-1762.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

In previous studies the 2A10 antigen of retinal pigment epithelium (RPE) was described. It is a cell-surface monoclonal antibody-defined antigen whose properties suggest a possible role in cell adhesive interactions. This report identified the 2A10 antigen as a beta 1 subunit of the cell-surface extracellular matrix receptor protein, integrin. Comparative biochemical, immunochemical, and histochemical analyses of the 2A10 antibody and other antibodies directed against the beta 1 subunit of integrin indicate similar tissue distributions, immunoblotting patterns, tryptic digestion profiles, and coimmunoprecipitation of the 2A10 antigen and the beta 1 subunit of integrin. The 2A10 antigen/beta 1 subunit differed in molecular weight in different tissues, with the RPE demonstrating a form intermediate between neural and endoderm-derived tissues. The application of this new antibody to functional studies of the role of integrin in RPE cell-substrate adhesion is described in the accompanying paper.

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