May 1991
Volume 32, Issue 6
Free
Articles  |   May 1991
Rapid deterioration of lens fibers in GSH-depleted mouse pups.
Author Affiliations
  • H I Calvin
    Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
  • C Medvedovsky
    Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
  • J C David
    Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
  • T M Broglio
    Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
  • J L Hess
    Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
  • S C Fu
    Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
  • B V Worgul
    Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
Investigative Ophthalmology & Visual Science May 1991, Vol.32, 1916-1924. doi:
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    • Get Citation

      H I Calvin, C Medvedovsky, J C David, T M Broglio, J L Hess, S C Fu, B V Worgul; Rapid deterioration of lens fibers in GSH-depleted mouse pups.. Invest. Ophthalmol. Vis. Sci. 1991;32(6):1916-1924.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Lens opacities developed within 48-72 hr in mice that received a series of eight injections of L-buthionine sulfoximine, a specific inhibitor of glutathione (GSH) biosynthesis, on postnatal days 8 and 9. Initial histopathologic features consisted of swollen fibers in the central anterior cortex and displacement of cell nuclei from the bow region to the posterior cortex. These aberrations suggest early fiber cell membrane and/or cytoskeletal dysfunction. A massive wave of fiber cell lysis then engulfed the entire lens cortex and nucleus within 24 hr and left only epithelial cells intact, suggesting a concerted mechanism of cataract generation. The acellular core of the mature cataract seen on postnatal day 16 consisted of a granular matrix in which pycnotic and fragmented cell nuclei were located near the terminus of the lens epithelium. The epithelium displayed increased mitotic activity and meridional row disorganization. During the next two weeks, rapid regeneration of lens fibers, displacement of the acellular necrotic cytoplasm to the center and rear of the lens, and vacuole formation were observed. As new fibers were differentiated, partial regeneration of the bow was seen. However, the cataract was irreversible.

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