September 1991
Volume 32, Issue 10
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Articles  |   September 1991
Gamma-interferon effects on human fibroblasts from Tenon's capsule.
Author Affiliations
  • M A Latina
    Wellman Laboratories, Massachusetts General Hospital, Boston 02114.
  • S J Belmonte
    Wellman Laboratories, Massachusetts General Hospital, Boston 02114.
  • C Park
    Wellman Laboratories, Massachusetts General Hospital, Boston 02114.
  • E Crean
    Wellman Laboratories, Massachusetts General Hospital, Boston 02114.
Investigative Ophthalmology & Visual Science September 1991, Vol.32, 2806-2815. doi:
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    • Get Citation

      M A Latina, S J Belmonte, C Park, E Crean; Gamma-interferon effects on human fibroblasts from Tenon's capsule.. Invest. Ophthalmol. Vis. Sci. 1991;32(10):2806-2815.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Gamma-interferon (G-IFN) exhibits antiproliferative effects, inhibits collagen synthesis by fibroblasts, and may help to regulate the turnover of fibrous tissue. Fibroblasts from Tenon's capsule are cellular components that contribute to unsuccessful glaucoma filtration surgery. Therefore, the authors studied the effects of G-IFN on growth, wound closure, collagen synthesis, and extracellular matrix in cultured human Tenon's capsule fibroblasts (HTCF). HTCF incubated with G-IFN at doses of 1 to 1000 U/ml showed cellular heterogeneity, with some cells showing morphologic changes characteristic of senescence, and a dose-dependent inhibition of wound closure. At 1000 U/ml, G-IFN reduced collagen synthesis by 57%, reduced immunofluorescent staining of collagen type I and fibronectin network, and altered the organization of actin microfilaments into large cable-like structures. Cell proliferation and viability were not affected at any concentration of G-IFN. These results suggest that G-IFN may be useful to modulate wound healing after filtration surgery.

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