April 1991
Volume 32, Issue 5
Free
Articles  |   April 1991
Transplantation of photoreceptors labeled with tritiated thymidine into RCS rats.
Author Affiliations
  • P Gouras
    Edward S. Harkness Eye Institute, Columbia University, New York, New York.
  • J Du
    Edward S. Harkness Eye Institute, Columbia University, New York, New York.
  • M Gelanze
    Edward S. Harkness Eye Institute, Columbia University, New York, New York.
  • R Kwun
    Edward S. Harkness Eye Institute, Columbia University, New York, New York.
  • H Kjeldbye
    Edward S. Harkness Eye Institute, Columbia University, New York, New York.
  • R Lopez
    Edward S. Harkness Eye Institute, Columbia University, New York, New York.
Investigative Ophthalmology & Visual Science April 1991, Vol.32, 1704-1707. doi:
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      P Gouras, J Du, M Gelanze, R Kwun, H Kjeldbye, R Lopez; Transplantation of photoreceptors labeled with tritiated thymidine into RCS rats.. Invest. Ophthalmol. Vis. Sci. 1991;32(5):1704-1707.

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Abstract

Tritiated thymidine was administered to newborn rats to label photoreceptors, about 50% of which are still dividing. These photoreceptors were enzymatically dissociated and separated from the remainder of the retina after the infant rat matured. These labeled photoreceptors were then transplanted into a foreign host retina in the region of the outer nuclear layer. The hosts were ocular, albinotic, Royal College of Surgeons (RCS) rats, congenic to the normal donors and at least 4 months old, a time when virtually all the photoreceptors have degenerated from their retinas. The transplant site was examined at various times after transplantation by light microscope autoradiography. Labeled photoreceptor cell bodies were found in clusters in the outer nuclear layer region for as long as 3 months after transplantation surgery.

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