October 1994
Volume 35, Issue 11
Free
Articles  |   October 1994
Expression of soluble phototransduction-associated proteins in ground squirrel retina.
Author Affiliations
  • M von Schantz
    Department of Zoology, University of Göteborg, Sweden.
  • A Szél
    Department of Zoology, University of Göteborg, Sweden.
  • T van Veen
    Department of Zoology, University of Göteborg, Sweden.
  • D B Farber
    Department of Zoology, University of Göteborg, Sweden.
Investigative Ophthalmology & Visual Science October 1994, Vol.35, 3922-3930. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M von Schantz, A Szél, T van Veen, D B Farber; Expression of soluble phototransduction-associated proteins in ground squirrel retina.. Invest. Ophthalmol. Vis. Sci. 1994;35(11):3922-3930.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: This study describes the expression and distribution of arrestin, phosducin, and recoverin in the cone-dominant retina of the ground squirrel Spermophilus tridecemlineatus. METHODS: mRNA expression was studied by blot hybridization of ground squirrel retinal RNA, with human and murine RNA as controls. The distribution of the gene products in the ground squirrel retina was investigated by immunocytochemistry using radial and consecutive tangential sections. RESULTS: Northern blot hybridization showed messages for arrestin (1.9 kb), phosducin (1.4 kb), and recoverin (1.2 kb) in ground squirrel retinal RNA. Both controls showed transcripts of the same or similar sizes. Rod-like cells and blue cones were stained by antibodies against arrestin and phosducin. The arrestin antiserum stained the whole cell bodies, most intensely in the myoid region, whereas phosducin immunoreactivity was confined to the outer and inner segments, which were stained with approximately equal intensity. The strongest immunoreaction was found in the photoreceptor plasma membrane. Recoverin antibodies recognized the entire soma of all photoreceptor cells. The myoid region and the synaptic pedicles were most heavily stained. No light-dependent migration was observed with either antiserum in any photoreceptor type. CONCLUSION: The presence of arrestin immunoreactivity in rod-like cells and blue cones is consistent with previous reports on other mammals. However, it has not been reported previously that phosducin immunoreactivity is distributed in the same way. The colocalization of arrestin and phosducin in rod-like cells and blue cones is yet another trait distinguishing blue cones from red and green cones.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×