October 1994
Volume 35, Issue 11
Free
Articles  |   October 1994
Analysis of electroretinogram during systemic hypercapnia with intraretinal K(+)-microelectrodes in cats.
Author Affiliations
  • K Hiroi
    Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
  • F Yamamoto
    Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
  • Y Honda
    Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
Investigative Ophthalmology & Visual Science October 1994, Vol.35, 3957-3961. doi:
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      K Hiroi, F Yamamoto, Y Honda; Analysis of electroretinogram during systemic hypercapnia with intraretinal K(+)-microelectrodes in cats.. Invest. Ophthalmol. Vis. Sci. 1994;35(11):3957-3961.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: Systemic hypercapnia alters the intraretinal pH and metabolism and profoundly affects the vitreal electroretinogram (ERG). Using intraretinal K(+)-selective microelectrodes (ISMs), the authors examined the effects of systemic hypercapnia on ERG in the intact cat eye in vivo. METHODS: Double-barreled K(+)-ISMs were used to record intraretinal, light-evoked [K+]o decrease, intraretinal ERG, vitreal ERG, and standing potential (SP) in the dark-adapted retina. Hypercapnia was induced by adding CO2 to the inhaled mixture of gas (without significant hypoxia). RESULTS: The standing potential was elevated, and the vitreal ERG c-wave increased in amplitude whereas the b-wave decreased during hypercapnia. Intraretinal recordings showed that the amplitudes of slow PIII and the transepithelial (TEP) c-wave decreased. The slow PIII decreased more than the TEP c-wave. The light-evoked [K+]o decrease also was reduced, but no change was noted in the subretinal [K+]o. CONCLUSION: The neural retina was more sensitive than the retinal pigment epithelium to the intraretinal acidification caused by systemic hypercapnia. However, the change in the SP and b-wave suggested complicated effects other than acidosis.

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