October 1994
Volume 35, Issue 11
Free
Articles  |   October 1994
Lectin electron microscopic histochemistry of the pseudoexfoliative material in the skin.
Author Affiliations
  • F Amari
    Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
  • S Nagata
    Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
  • J Umihira
    Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
  • M Nohara
    Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
  • N Usuda
    Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
  • K Segawa
    Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
Investigative Ophthalmology & Visual Science October 1994, Vol.35, 3962-3966. doi:
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    • Get Citation

      F Amari, S Nagata, J Umihira, M Nohara, N Usuda, K Segawa; Lectin electron microscopic histochemistry of the pseudoexfoliative material in the skin.. Invest. Ophthalmol. Vis. Sci. 1994;35(11):3962-3966.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To investigate the hypothesis that pseudoexfoliation (PSX) syndrome is a systemic disorder, the authors studied the composition of glycoconjugates in the intraocular and extraocular PSX material at the electron microscopic level, using a panel of lectins as cytochemical probes. METHODS: The authors examined 8 lid skins, 11 trabecular tissues, and 3 cataractous lenses from human eyes with PSX syndrome. Tissues were processed for electron microscopical histochemistry and stained with PNA, RCA120, DBA, SBA, ConA, WGA, UEA-I, and Lotus, with an indirect lectin-colloidal gold technique. RESULTS: Both the intraocular and extraocular PSX materials manifested almost identical reactivity to lectins, which indicated that glycoconjugates in the PSX material contained with sugar residues of galactose (PNA, RCA120), alpha-mannose (ConA), and N-acetyl-D-glucosamine (WGA). On the other hand, it was indicated that sugar residues of N-acetyl-D-galactosamine (DBA, SBA) and fucose (UEA-I, Lotus) were absent. Granular inclusions and microfibrils in the capsule and ocular zonules were stained similarly and weakly. CONCLUSIONS: The intraocular and extraocular PSX materials contained the same sugar residues of glycoconjugates, which suggested that those materials had the same nature. This study, the first documentation of lectin-binding sites on the extraocular PSX material, supported the hypothesis of PSX syndrome as a systemic disorder.

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