December 1994
Volume 35, Issue 13
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Articles  |   December 1994
Distribution of an endogenous 16-kd S-lac lectin in the chicken retina.
Author Affiliations
  • L F Castagna
    Department of Biological Chemistry, Faculty of Chemical Sciences, Universidad Nacional de Cordoba, Argentina.
  • C A Landa
    Department of Biological Chemistry, Faculty of Chemical Sciences, Universidad Nacional de Cordoba, Argentina.
Investigative Ophthalmology & Visual Science December 1994, Vol.35, 4310-4316. doi:
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      L F Castagna, C A Landa; Distribution of an endogenous 16-kd S-lac lectin in the chicken retina.. Invest. Ophthalmol. Vis. Sci. 1994;35(13):4310-4316.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To examine by indirect immunofluorescence the distribution of an endogenous 16-kd S-lac lectin (soluble lactose binding lectin) during development of the chicken retina. METHODS: Cryosections of retinal tissue at different developmental stages and cultured retinal cells (either not permeabilized or permeabilized with acetone) were incubated with a rabbit antiserum that specifically reacts with the retinal 16-kd S-lac lectin. After incubation with a fluorescent-labeled secondary antibody, tissue sections and cultured cells were analyzed by fluorescence microscopy. RESULTS: Retina was weakly stained with the antiserum on early embryonic day 7, whereas on embryonic days 13 and 18 it showed a restricted "granular" staining in the outer retina. At embryonic day 18, in addition, there was widespread staining in all retinal layers. This pattern was maintained by postnatal day 5 and in the adult retina, although the intensity of the staining of the outer retina was weaker. In retinal cell cultures, glial-like flat cells and monopolar, bipolar, and multipolar neurons were stained with the antiserum, but only if they had been previously permeabilized with acetone. CONCLUSION: The results suggest that the distribution of a 16-kd S-lac lectin changes during retinal development. Cell culture experiments indicate that most often the lectin is localized intracellularly in the different retinal cell types.

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