June 1994
Volume 35, Issue 7
Free
Articles  |   June 1994
Activin expression by cultured human retinal pigment epithelial cells.
Author Affiliations
  • G J Jaffe
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710.
  • C E Harrison
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710.
  • G M Lui
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710.
  • W L Roberts
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710.
  • P C Goldsmith
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710.
  • S Mesiano
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710.
  • R B Jaffe
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710.
Investigative Ophthalmology & Visual Science June 1994, Vol.35, 2924-2931. doi:
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    • Get Citation

      G J Jaffe, C E Harrison, G M Lui, W L Roberts, P C Goldsmith, S Mesiano, R B Jaffe; Activin expression by cultured human retinal pigment epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1994;35(7):2924-2931.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To determine whether human retinal pigment epithelial (hRPE) cells produce activin, a growth factor in the transforming growth factor beta family, and to characterize growth regulatory effects of activin on retinal pigment epithelium. METHODS: mRNA expression was examined using polymerase chain reaction with primers specific for the beta A and beta B chains of activin and by slot blot analysis with a probe specific for the beta A chain. Protein localization was determined immunocytochemically using antibodies specific for the beta A chain of activin and intact activin A. The effect of activin A on DNA synthesis was studied by measuring (3H) thymidine incorporation after cells were exposed to recombinant human activin A (rhA). Growth regulatory effects of rhA on hRPE cells were examined with cell growth assays. RESULTS: beta A mRNA was expressed constitutively in 8/8 cells lines tested. beta B mRNA was not expressed in any of the six cell lines tested but was expressed in human ovarian granulosa cell controls. Positive immunostaining was observed for both the beta A chain and intact activin A. (3H) thymidine incorporation was inhibited 44% (P < 0.025), 45% (P < 0.025), and 44% (P < 0.015) when RPE cells were exposed to 100 ng/ml rhA and grown in serum-free medium, medium with 0.5% serum, and 1% serum, respectively. Cell growth was inhibited 33.2% (P = 0.0001) after RPE cells were exposed to 100 ng/ml rhA for 8 days. CONCLUSIONS: These results suggest that activin A can act as an autocrine-paracrine growth regulator in RPE cells and may help control cellular growth in ocular development and proliferative eye disease.

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