August 1994
Volume 35, Issue 9
Free
Articles  |   August 1994
Inhibitory effect of TGF-beta 2 in human aqueous humor on bovine lens epithelial cell proliferation.
Author Affiliations
  • D Kurosaka
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
  • T Nagamoto
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Investigative Ophthalmology & Visual Science August 1994, Vol.35, 3408-3412. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      D Kurosaka, T Nagamoto; Inhibitory effect of TGF-beta 2 in human aqueous humor on bovine lens epithelial cell proliferation.. Invest. Ophthalmol. Vis. Sci. 1994;35(9):3408-3412.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To determine whether transforming growth factor-beta 2 (TGF-beta 2) in human aqueous humor inhibits the proliferation of lens epithelial cells. METHODS: Bovine lens epithelial cells (BLECs) obtained from the central zone of the anterior capsule were cultured in complete medium (F-12 nutrient mixture supplemented with 5% fetal bovine serum). Human aqueous humor was obtained from 11 patients during cataract surgery. Medium was removed and replaced with medium containing various concentrations of porcine platelet-derived TGF-beta 2 or 10% human aqueous humor, with or without anti-TGF-beta 2 neutralizing antibody. The number of cells was counted at 96 hours. RESULTS: Porcine platelet-derived TGF-beta 2 inhibited BLEC proliferation in a dose-dependent manner. Anti-TGF-beta 2 antibody blocked the inhibitory effect of TGF-beta 2 on BLEC proliferation. The 10% human aqueous humor stimulated the proliferation of BLECs. Addition of anti-TGF-beta 2 antibody to the 10% human aqueous humor significantly enhanced this stimulatory effect on BLEC proliferation. CONCLUSIONS: TGF-beta 2 in human aqueous humor inhibits the proliferation of lens epithelial cells in vitro.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×