July 1994
Volume 35, Issue 8
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Articles  |   July 1994
Distribution of FGF-5 in the rhesus macaque retina.
Author Affiliations
  • T Kitaoka
    Department of Ophthalmology, University of California, Davis 95616.
  • A E Aotaki-Keen
    Department of Ophthalmology, University of California, Davis 95616.
  • L M Hjelmeland
    Department of Ophthalmology, University of California, Davis 95616.
Investigative Ophthalmology & Visual Science July 1994, Vol.35, 3189-3198. doi:
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      T Kitaoka, A E Aotaki-Keen, L M Hjelmeland; Distribution of FGF-5 in the rhesus macaque retina.. Invest. Ophthalmol. Vis. Sci. 1994;35(8):3189-3198.

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Abstract

PURPOSE: To determine the distribution of mRNA transcribed from the FGF-5 gene and the distribution of FGF-5 protein in the normal adult retina of the rhesus macaque. METHODS: Freshly enucleated globes from rhesus macaque were prepared for Northern blot analysis, in situ hybridization, and immunohistochemical studies. A 350 base pair sequence from the human FGF-5 was subcloned and used to prepare 35S-labeled cRNAs for Northern and in situ experiments. Antipeptide antibodies were raised against an aminoterminal sequence as well as an internal sequence and were affinity purified on recombinant FGF-5 coupled to AffiGel-10. The specificity of antibody probes was verified by dot blot and Western blot analyses. RESULTS: Ganglion cells and photoreceptors express the highest levels of FGF-5 mRNA, although all neurons and the retinal pigment epithelium (RPE) were clearly labeled. Immunohistochemical staining similarly revealed a widespread distribution of the protein, with prominent labeling observed in photoreceptor inner segments, especially in cone cells. Plexiform layers and RPE cells were also clearly labeled. CONCLUSIONS: Clear evidence was established for the expression of FGF-5 in the retina, where it may play an important extracellular role as a secreted member of the FGF gene family.

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