August 1994
Volume 35, Issue 9
Free
Articles  |   August 1994
Characterization of intraocular tumors arising in transgenic mice.
Author Affiliations
  • R Anand
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
  • D Ma
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
  • H Alizadeh
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
  • S A Comerford
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
  • J F Sambrook
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
  • M J Gething
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
  • I W McLean
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
  • J Y Niederkorn
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
Investigative Ophthalmology & Visual Science August 1994, Vol.35, 3533-3539. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      R Anand, D Ma, H Alizadeh, S A Comerford, J F Sambrook, M J Gething, I W McLean, J Y Niederkorn; Characterization of intraocular tumors arising in transgenic mice.. Invest. Ophthalmol. Vis. Sci. 1994;35(9):3533-3539.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To characterize intraocular tumors that arise by in situ transformation in the choroid-retinal pigment epithelium (RPE) in transgenic mice bearing the SV40 oncogene under the control of the mouse tyrosinase promoter. METHODS: Tumors from TySV40 transgenic mice were characterized in vivo and in vitro by immunohistology, compound microscopy, and electron microscopy. Tumor cell lines were established and characterized for growth and metastatic potential in the eyes of nude mice. RESULTS: On light microscopy, ocular tumors were predominantly epithelioid, although occasional clusters of spindle cells were also present. Transmission electron microscopy revealed the presence of numerous basal infoldings and abundant multilaminated basement membranes on the ocular tumors. Tumors stained with antibodies to melanoma-associated antigens, gangliosides GD2 and GD3, and the SV40 T antigen. Radiolabeled transgenic tumor cells preferentially localized in the liver after intravenous injection in normal mice. Intracamerally transplanted transgenic tumors metastasized from the eyes to the livers of nude mice. CONCLUSIONS: In TySV40 transgenic mice, intraocular tumors develop that arise at the choroid-RPE interface, and they display morphologic and ultrastructural features consistent with RPE carcinomas. However, the transgenic tumors express melanoma-associated antigens and a propensity to metastasize to the liver, two features characteristic of uveal melanomas. The TySV40 transgenic murine tumors represent potentially useful tools for investigations into the biology and metastasis of intraocular neoplasms.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×