July 1994
Volume 35, Issue 8
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Articles  |   July 1994
Recoverin is highly uveitogenic in Lewis rats.
Author Affiliations
  • I Gery
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
  • N P Chanaud, 3rd
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
  • E Anglade
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
Investigative Ophthalmology & Visual Science July 1994, Vol.35, 3342-3345. doi:
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      I Gery, N P Chanaud, E Anglade; Recoverin is highly uveitogenic in Lewis rats.. Invest. Ophthalmol. Vis. Sci. 1994;35(8):3342-3345.

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Abstract

PURPOSE: Recoverin, a calcium-binding protein that selectively localizes to the retina and pineal gland, has been identified as the target for the putative pathogenic autoimmune process of cancer-associated retinopathy (CAR). The present study was aimed at testing the capacity of recoverin to induce experimental autoimmune uveoretinitis and pinealitis in Lewis rats. METHODS: Lewis rats were immunized against recombinant myristoylated recoverin by a single footpad injection of the protein, at various doses, emulsified in complete Freund's adjuvant. Development of uveoretinitis was monitored by clinical and histologic examinations, whereas pinealitis was detected by histologic examination. RESULTS: Immunization with recoverin induced severe panuveitic changes that closely resemble those induced by S-antigen (arrestin). The effect was dose-dependent, with 10 micrograms/rat the lowest immunopathogenic dose. Rats immunized with recoverin also developed pineal inflammation. CONCLUSION: Recoverin is highly immunopathogenic in Lewis rats. Although the ocular changes induced in rats differ from those seen in CAR, the data recorded here are in line with the concept that recoverin can initiate pathogenic autoimmune processes in the eye.

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