August 1995
Volume 36, Issue 9
Free
Articles  |   August 1995
Alpha-2 adrenergic receptors in the bovine retina. Presence of only the alpha-2D subtype.
Author Affiliations
  • J R Berlie
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha 68198-6260, USA.
  • L J Iversen
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha 68198-6260, USA.
  • H S Blaxall
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha 68198-6260, USA.
  • M E Cooley
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha 68198-6260, USA.
  • D M Chacko
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha 68198-6260, USA.
  • D B Bylund
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha 68198-6260, USA.
Investigative Ophthalmology & Visual Science August 1995, Vol.36, 1885-1892. doi:
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      J R Berlie, L J Iversen, H S Blaxall, M E Cooley, D M Chacko, D B Bylund; Alpha-2 adrenergic receptors in the bovine retina. Presence of only the alpha-2D subtype.. Invest. Ophthalmol. Vis. Sci. 1995;36(9):1885-1892.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To identify and characterize the alpha-2 adrenergic receptor subtypes present in the bovine neurosensory retina. METHODS: Radioligand saturation and inhibition binding assays were performed with the antagonist radioligands [3H]RX821002 and [3H]rauwolscine. RESULTS: [3H]RX821002 bound to a single class of receptors with the characteristics of an alpha-2 adrenergic receptor with an affinity (KD) of 0.16 nM and a receptor density (Bmax) of 1500 fmol/mg protein. Correlation of the affinities (pKi values) for nine antagonists in the bovine neurosensory retina with the alpha-2D receptor of the bovine pineal gave a correlation coefficient of 0.99. The correlation coefficients for the alpha-2A (0.84), alpha-2B (0.36), and alpha-2C (0.39) subtypes were much lower. The presence of a minor population of alpha-2B or alpha-2C receptors was excluded. CONCLUSIONS: A high density of alpha-2D adrenergic receptors is present in the bovine neurosensory retina. Neither the alpha-2B nor the alpha-2C subtype is detectable.

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