October 1997
Volume 38, Issue 11
Free
Articles  |   October 1997
Astrocytes increase barrier properties and ZO-1 expression in retinal vascular endothelial cells.
Author Affiliations
  • T W Gardner
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
  • E Lieth
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
  • S A Khin
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
  • A J Barber
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
  • D J Bonsall
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
  • T Lesher
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
  • K Rice
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
  • W A Brennan, Jr
    Department of Ophthalmology, Penn State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Investigative Ophthalmology & Visual Science October 1997, Vol.38, 2423-2427. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T W Gardner, E Lieth, S A Khin, A J Barber, D J Bonsall, T Lesher, K Rice, W A Brennan; Astrocytes increase barrier properties and ZO-1 expression in retinal vascular endothelial cells.. Invest. Ophthalmol. Vis. Sci. 1997;38(11):2423-2427.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: Diabetic retinopathy and other diseases associated with retinal edema are characterized by increased microvascular leakage. Astrocytes have been proposed to maintain endothelial function in the brain, suggesting that glial impairment may underlie the development of retinal edema. The purpose of this study was to test the effects of astrocytes on barrier properties in retinal microvascular endothelial cells. METHODS: Bovine retinal microvascular endothelial cells were exposed to conditioned media from rat brain astrocytes. Transendothelial electrical resistance (TER) was determined on 24-mm Transwell (Cambridge, MA) polycarbonate filters with the End-Ohm device (World Precision Instruments, Sarasota, FL). ZO-1 protein content was quantified by microtiter enzyme-linked immunosorbent assay. RESULTS: Astrocyte-conditioned medium (ACM) significantly increased TER (P < 0.0001) and ZO-1 content (P < 0.01). Both serum-containing and serum-free N1B defined ACM increased ZO-1 expression, but heating abolished the effect. Serum-free ACM decreased cell proliferation by 16%. CONCLUSIONS: Astrocytes release soluble, heat-labile factors that increase barrier properties and tight junction protein content. These results suggest that astrocytes enhance blood-retinal barrier properties, at least in part by increasing tight junction protein expression. Our findings suggest that glial malfunction plays an important role in the pathogenesis of vasogenic retinal edema.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×