November 1997
Volume 38, Issue 12
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Articles  |   November 1997
Localization of platelet-activating factor receptor messenger RNA in the rat eye.
Author Affiliations
  • M Mori
    Department of Ophthalmology, Faculty of Medicine, University of Tokyo, Japan. mmori-tky@umin.ac.jp
  • M Aihara
    Department of Ophthalmology, Faculty of Medicine, University of Tokyo, Japan. mmori-tky@umin.ac.jp
  • T Shimizu
    Department of Ophthalmology, Faculty of Medicine, University of Tokyo, Japan. mmori-tky@umin.ac.jp
Investigative Ophthalmology & Visual Science November 1997, Vol.38, 2672-2678. doi:
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      M Mori, M Aihara, T Shimizu; Localization of platelet-activating factor receptor messenger RNA in the rat eye.. Invest. Ophthalmol. Vis. Sci. 1997;38(12):2672-2678.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To determine the distribution of platelet-activating factor (PAF) receptor in the rat eye. METHODS: Platelet-activating factor receptor messenger RNA (mRNA) expression was evaluated with reverse transcription-polymerase chain reaction (RT-PCR) in RNAs from several rat ocular tissues. Distribution of the expression was determined with in situ hybridization in adult rat eye sections and a 35S-labeled complementary RNA probe synthesized from the rat PAF receptor complementary DNA. In situ hybridization was also done with sections immunostained with OX-42, a microglia marker. RESULTS: RT-PCR revealed that levels of PAF receptor expression were similar among the ocular tissues studied. The in situ hybridization signals were found in the corneal epithelium, iris and ciliary body, and ganglion and microglial cells in the retina. CONCLUSIONS: Platelet-activating factor receptor mRNA was ubiquitously expressed in the rat eye with relative concentration in the corneal epithelium, iris and ciliary body, and retinal ganglion and microglial cells.

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