November 1997
Volume 38, Issue 12
Free
Articles  |   November 1997
Differential expression of maf-1 and maf-2 genes in the developing rat lens.
Author Affiliations
  • K Yoshida
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • J Imaki
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • Y Koyama
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • T Harada
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • Y Shinmei
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • C Oishi
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • Y Matsushima-Hibiya
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • A Matsuda
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • S Nishi
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • H Matsuda
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
  • M Sakai
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
Investigative Ophthalmology & Visual Science November 1997, Vol.38, 2679-2683. doi:
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    • Get Citation

      K Yoshida, J Imaki, Y Koyama, T Harada, Y Shinmei, C Oishi, Y Matsushima-Hibiya, A Matsuda, S Nishi, H Matsuda, M Sakai; Differential expression of maf-1 and maf-2 genes in the developing rat lens.. Invest. Ophthalmol. Vis. Sci. 1997;38(12):2679-2683.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To examine the expression of maf-1 and maf-2 protocogenes in the developing rat lens. METHODS: Maf-1 and maf-2 transcripts were assayed in rat lenses on embryonic days 13 and 16 (E13 and E16) by in situ hybridization using single-stranded RNA probes. Proteins encoded by the maf-2 gene were assayed immunocytochemically in embryonic (E12, 13, 16, 19) and postnatal day 14 and 90 (P14 and P90) lenses. RESULTS: In embryonic lenses, we detected maf-1 messenger RNA (mRNA) in the lens epithelium and maf-2 mRNA diffusely distributed in the lens fiber cells. By immunocytochemistry, Maf-2 was detected on E12 in the nuclei of almost all lens pit cells. On days E13, E16, and E19, however, lens epithelial cells showed no immunoreactivity, but nuclei of fiber cells reacted strongly. On P14, nuclei containing Maf-2 protein were confined to the equator of the lens, but at 3 months of age, no Maf-2 could be detected in the rat lens. Western blotting showed that the anti-Maf-2 antiserum reacted with a single protein, of molecular weight approximately 39 kDa, in rat lens. CONCLUSIONS: Results showed the spatial and temporal regulation of maf gene expression and suggest that these genes participate in transcriptional regulation during the development of the lens in the rat.

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