June 1997
Volume 38, Issue 7
Free
Articles  |   June 1997
Muscarinic receptor subtypes in human iris-ciliary body measured by immunoprecipitation.
Author Affiliations
  • D W Gil
    Department of Biological Sciences, Allergan, Inc., Irvine CA 92612, USA.
  • H A Krauss
    Department of Biological Sciences, Allergan, Inc., Irvine CA 92612, USA.
  • A M Bogardus
    Department of Biological Sciences, Allergan, Inc., Irvine CA 92612, USA.
  • E WoldeMussie
    Department of Biological Sciences, Allergan, Inc., Irvine CA 92612, USA.
Investigative Ophthalmology & Visual Science June 1997, Vol.38, 1434-1442. doi:
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    • Get Citation

      D W Gil, H A Krauss, A M Bogardus, E WoldeMussie; Muscarinic receptor subtypes in human iris-ciliary body measured by immunoprecipitation.. Invest. Ophthalmol. Vis. Sci. 1997;38(7):1434-1442.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To determine the relative levels of the five muscarinic receptor subtypes in the anterior segment of the human eye. METHODS: Antisera selective for each of the five muscarinic receptor proteins were incubated with [3H]-QNB bound receptors solubilized from human iris sphincter, ciliary muscle, and ciliary processes. Precipitation of the radiolabeled receptor-antibody complexes and scintillation counting enabled quantitation of the subtypes in the various tissues. Reverse transcription-polymerase chain reaction was performed on the tissues and cultured smooth muscle cells derived from them. RESULTS: Approximately 60% to 75% of the muscarinic receptors in the human iris sphincter and ciliary body are the m3 subtype. Lower levels (5% to 10%) of the m2 and m4 receptors are present in these tissues. The m1 receptor (7%) was detected in the ciliary processes and iris sphincter and the m5 receptor (5%), which is usually found only in the central nervous system, was present in the iris sphincter. CONCLUSIONS: The m3 subtype is the predominant muscarinic receptor in the anterior segment of the human eye. The extensive heterogeneity of muscarinic receptors makes it difficult to predict whether subtype-selective drugs will have an improved efficacy and side-effect profile.

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