February 1997
Volume 38, Issue 2
Free
Articles  |   February 1997
cis-Hydroxyproline inhibits proliferation, collagen synthesis, attachment, and migration of cultured bovine retinal pigment epithelial cells.
Author Affiliations
  • J S Yoo
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
  • T Sakamoto
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
  • C Spee
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
  • H Kimura
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
  • M S Harris
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
  • D R Hinton
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
  • E P Kay
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
  • S J Ryan
    Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles 90033, USA.
Investigative Ophthalmology & Visual Science February 1997, Vol.38, 520-528. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J S Yoo, T Sakamoto, C Spee, H Kimura, M S Harris, D R Hinton, E P Kay, S J Ryan; cis-Hydroxyproline inhibits proliferation, collagen synthesis, attachment, and migration of cultured bovine retinal pigment epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1997;38(2):520-528.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: Proliferative vitreoretinopathy (PVR) is characterized by the proliferation and migration of retinal pigment epithelial (RPE) and other cells into the vitreous cavity. The PVR membrane formation also is associated with collagen production by RPE. The authors examined the effect of a proline analog, cis-hydroxyproline (CHP), on proliferation, collagen synthesis, attachment, and migration of bovine RPE in vitro. METHODS: The effect of CHP on cell proliferation was determined as a function of dosage and days in culture by counting the cell numbers on days 3, 6, and 9. Collagen synthesis was determined by trichloroacetic acid precipitation of the radiolabeled samples before and after bacterial collagenase digestion. The attachment assay involved type I collagen or fibronectin substrates or both (2.5 micrograms/well). For migration experiments, RPE cells were removed from a defined area of a confluent culture, and migration was quantitated by counting the number of cells migrating into the denuded area over 30 hours. RESULTS: The addition of CHP inhibited RPE proliferation in both a dose- and a time-dependent manner; collagen synthesis, attachment, and migration also were inhibited by CHP in a dose-dependent manner. When the culture plates were coated with collagen, < 100 micrograms/ml of CHP had no effect on cell attachment. Higher doses of CHP resulted in mild inhibition of attachment on collagen-coated plates. Simultaneous addition of L-proline to the cultures resulted in blockade of these inhibitory effects on proliferation, collagen synthesis, attachment, and migration. CONCLUSIONS: The results show that RPE functions critical to the development of PVR are inhibited by CHP, suggesting the possibility that this drug may have potential clinical application.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×