February 1997
Volume 38, Issue 2
Free
Articles  |   February 1997
The antigen-bearing eye and the spleen are indispensable in maintaining anterior chamber-associated immune deviation.
Author Affiliations
  • Y F Yao
    Department of Ophthalmology, Osaka University Medical School, Japan.
  • Y Inoue
    Department of Ophthalmology, Osaka University Medical School, Japan.
  • D Miyazaki
    Department of Ophthalmology, Osaka University Medical School, Japan.
  • Y Hara
    Department of Ophthalmology, Osaka University Medical School, Japan.
  • Y Shimomura
    Department of Ophthalmology, Osaka University Medical School, Japan.
  • Y Tano
    Department of Ophthalmology, Osaka University Medical School, Japan.
  • Y Ohashi
    Department of Ophthalmology, Osaka University Medical School, Japan.
Investigative Ophthalmology & Visual Science February 1997, Vol.38, 534-539. doi:
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    • Get Citation

      Y F Yao, Y Inoue, D Miyazaki, Y Hara, Y Shimomura, Y Tano, Y Ohashi; The antigen-bearing eye and the spleen are indispensable in maintaining anterior chamber-associated immune deviation.. Invest. Ophthalmol. Vis. Sci. 1997;38(2):534-539.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To investigate the role of the eye and the spleen in maintaining suppression of delayed-type hypersensitivity (DTH) after anterior chamber (AC) inoculation of allogeneic splenocytes. METHODS: Suppression of DTH response was tested in BALB/c mice after AC inoculation of allogeneic B10.D2 splenocytes. Seven days after AC injection, the antigen-inoculated eyes were enucleated or the spleens were removed. After enculeation or splenectomy at different time intervals, DTH responses in groups of the BALB/c mice were examined. Spleen components obtained from BALB/c mice that had been primed by B10.D2 splenocytes in the AC 7 days earlier were transferred intravenously to groups of naive syngeneic acceptors. At various intervals after adoptive transfer, variations of DTH responses were tested. RESULTS: Inoculation of B10.D2 splenocytes to the AC of BALB/c mice induced antigen-specific suppression of DTH. Either enucleation of the antigen-inoculated eyes or splenectomy weakened the DTH-suppressive effect within 5 weeks and abolished it within 9 weeks, whereas the mice retaining both antigen-inoculated eyes and spleens maintained longstanding DTH suppression. Adoptive transfer of spleen components to syngeneic acceptors demonstrated DTH suppression for only 3 weeks. CONCLUSIONS: The antigen-inoculated eye and spleen are required for long-standing suppression of DTH after AC inoculation of allogeneic splenocytes.

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