July 1997
Volume 38, Issue 8
Free
Articles  |   July 1997
Treatment of bleb leaks with transforming growth factor-beta in the rabbit model.
Author Affiliations
  • J W Doyle
    Department of Ophthalmology, University of Florida, Gainisville 32610-0284, USA.
  • M F Smith
    Department of Ophthalmology, University of Florida, Gainisville 32610-0284, USA.
  • J A Garcia
    Department of Ophthalmology, University of Florida, Gainisville 32610-0284, USA.
  • G Schultz
    Department of Ophthalmology, University of Florida, Gainisville 32610-0284, USA.
  • M B Sherwood
    Department of Ophthalmology, University of Florida, Gainisville 32610-0284, USA.
Investigative Ophthalmology & Visual Science July 1997, Vol.38, 1630-1634. doi:
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    • Get Citation

      J W Doyle, M F Smith, J A Garcia, G Schultz, M B Sherwood; Treatment of bleb leaks with transforming growth factor-beta in the rabbit model.. Invest. Ophthalmol. Vis. Sci. 1997;38(8):1630-1634.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: The mechanism through which peribleb injection of autologous blood results in resolution of bleb leak in the rabbit model remains unclear. This study evaluates the clinical and histologic effects of peribleb injection of transforming growth factor-beta (TGF-beta) after leak induction in mitomycin-C-treated blebs. METHOD: Posterior lip sclerectomies treated with mitomycin-C were created in New Zealand White rabbits. On postoperative day 7, a standardized stab incision was performed on all blebs, and the eyes were randomized to receive a peribleb injection of either TGF-beta or of a balanced salt solution. RESULTS: Injection of TGF-beta was associated with the resolution of bleb leak and maintenance of a functioning bleb in 50% (4 of 8) of treated eyes. The remaining TGF-beta-treated eyes and control eyes demonstrated continued bleb leaks or bleb failures with intraocular pressure returning to preoperative levels. Histologic examination revealed increased peribleb cellularity and denser collagen deposition in the TGF-beta-treated eyes compared with that observed in control eyes. CONCLUSIONS: Peribleb TGF-beta injections may contribute to healing bleb leaks, but the injections do not appear in this model to be as useful as whole-blood injections.

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