Figure 2Eillustrates the longitudinal changes in the magnitude of refractive astigmatism (mean ± SEM) of the treated eyes for control and treatment groups. Compared to the control group, significantly higher magnitudes of astigmatism were found in the treated eyes during the 7-day treatment period in all treatment groups (repeated-measures ANOVA,
P < 0.01; Dunnett post hoc test, all
P < 0.05). In addition, 28 (74%) of the 38 treated birds had the highest magnitude of astigmatism (peak astigmatism) near the middle of the treatment period (representative animals are marked with asterisks in
Figs. 2A 2B 2C 2D ). This point is illustrated in plots of the average magnitudes of refractive astigmatism in the treated eyes in
Figure 2Fat three time points for each group of birds. The three time points were at the onset of the experiment (initial), when the highest magnitude of astigmatism occurred during the treatment period (peak data, excluding measurements at initial and end), and at the end of the treatment period (end). Repeated-measures ANOVA indicated that refractive astigmatism in the treated eyes were significantly different at these three time points for all groups of birds (including control animals, all
P < 0.01). The results of a post hoc test with Bonferroni correction indicated that, except for two comparisons marked as nonsignificant (ns) in
Fig 2F , the magnitude of astigmatism at peak period and those at the other two time points were significantly different (
P = 0.001–0.03). The exceptions were the comparison between initial and peak in the control group (
P = 0.62), and the comparison between peak and end in the diffuser group (
P = 0.20). Regardless, at the end of the treatment period, all treated groups had significantly higher magnitudes of refractive astigmatism in the treated eyes than did the control group (one-way ANOVA,
P < 0.001; Tukey post hoc test, all
P < 0.05). Furthermore, the magnitude of astigmatism at the end of the treatment period was significantly higher in chicks treated with diffusers than in those in the other three treatment groups (Tukey post hoc test, all
P < 0.05), but no significant differences in the magnitude of astigmatism were found between groups treated with −10, +10 D and constant light (Tukey post hoc test, all
P > 0.05).