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Mahnaz Shahidi, Justin Wanek, Norman P. Blair, Marek Mori; Three-Dimensional Mapping of Chorioretinal Vascular Oxygen Tension in the Rat. Invest. Ophthalmol. Vis. Sci. 2009;50(2):820-825. doi: 10.1167/iovs.08-2343.
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purpose. An optical section phosphorescence lifetime imaging system was developed for three-dimensional mapping of oxygen tension (Po 2) in chorioretinal vasculatures.
methods. A laser line was projected at an oblique angle and scanned on the retina after intravenous injection of an oxygen-sensitive molecular probe to generate phosphorescence optical section images. An automated software algorithm segmented and combined images from spatially adjacent locations to construct depth-displaced en face retinal images. Intravascular Po 2 was measured by determining the phosphorescence lifetime. Three-dimensional chorioretinal Po 2 maps were generated in rat eyes under varying fractions of inspired oxygen.
results. Under an air-breathing condition, mean Po 2 in the choroid, retinal arteries, capillaries, and veins were 58 ± 2 mm Hg, 47 ± 2 mm Hg, 44 ± 2 mm Hg, and 35 ± 2 mm Hg, respectively. The mean arteriovenous Po 2 difference was 12 ± 2 mm Hg. With a lower fraction of inspired oxygen, chorioretinal vascular Po 2 and mean arteriovenous Po 2 differences decreased compared with measurements under an air-breathing condition. Retinal venous Po 2 was statistically lower than Po 2 measured in the retinal artery, capillaries, and choroid (P < 0.004).
conclusions. Three-dimensional mapping of chorioretinal oxygen tension allowed quantitative Po 2 measurements in large retinal blood vessels and in retinal capillaries. This method has the potential to facilitate better understanding of retinal oxygenation in health and disease.
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