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Heather L. Chandler, Kathleen S. Reuter, Loraine T. Sinnott, Jason J. Nichols; Prevention of UV-Induced Damage to the Anterior Segment Using Class I UV-Absorbing Hydrogel Contact Lenses. Invest. Ophthalmol. Vis. Sci. 2010;51(1):172-178. doi: 10.1167/iovs.09-3996.
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Purpose. To determine whether class I ultraviolet (UV) light–blocking contact lenses prevent UV-induced pathologic changes in a rabbit model.
Methods. Twelve rabbits were assigned to 1 of 3 treatment groups (n = 4), as follows: senofilcon A (class I UV blocking) contact lenses; lotrafilcon A contact lenses (no reported UV blocking); no contact lens. The contralateral eye was patched without a contact lens. Animals received UV-B (1.667 J/cm2) exposure daily for 5 days. Postmortem tissue was examined as follows: in the cornea, the expression of matrix-metalloproteinases (MMPs) was evaluated by zymography, and apoptosis was evaluated by TUNEL and caspase-3 ELISA; ascorbate in the aqueous humor was evaluated by nuclear magnetic resonance spectroscopy; crystalline lens apoptosis was evaluated by TUNEL and caspase-3 ELISA.
Results. Exposed corneas showed a significant increase in MMP-2 and -9, TUNEL-positive cells, and caspase-3 activity in the lotrafilcon A group compared with the senofilcon A group (all P = 0.03). A significant decrease in aqueous humor ascorbate was observed in the exposed lotrafilcon A lens–wearing group compared with the exposed senofilcon A lens–wearing group (P = 0.03). Exposed crystalline lenses had significantly increased caspase-3 activity in the lotrafilcon A group compared with the senofilcon A group (P = 0.03). Increased numbers of TUNEL-positive cells were noted in both the lotrafilcon A and the non–contact lens groups.
Conclusions. The authors show that senofilcon A class I UV-blocking contact lenses are capable of protecting the cornea, aqueous humor, and crystalline lens of rabbits from UV-induced pathologic changes.
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