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Deyrick Osmond Dean, Carol Riley Kent, Michael Tytell; Constitutive and Inducible Heat Shock Protein 70 Immunoreactivity in the Normal Rat Eye. Invest. Ophthalmol. Vis. Sci. 1999;40(12):2952-2962.
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purpose. Distributions for the constitutive and inducible 70-kDa heat shock
proteins, Hsc70 and Hsp70, in different parts of the rat eye are likely
to be related to the metabolic demands required for absorption and
detection of light. This study was conducted to better
understand the functions of Hsc70 and Hsp70 in these tissues and to
provide a basis for elucidating their contributions to the maintenance
and repair of ocular structures subsequent to tissue injury or cellular
methods. Eyes from male Sprague–Dawley rats (200–300 g) were fixed in
methacarn and embedded in paraffin. Sagittal sections (10 μm) through
the optic nerve were stained with hematoxylin and eosin or incubated
with heat shock protein antibody or control IgG. Bound antibody was
visualized using an avidin–biotin–horseradish peroxidase detection
results. Hsc70 immunoreactivity was detected in all layers of the retina, except
the outer segments. In the retinal pigment epithelium, staining was
restricted to cells near the optic nerve–retina junction. Intense
staining was also observed in glial nuclei of the optic nerve, whereas
weaker staining was observed in the basal and wing cells of the limbal
and corneal epithelia. In contrast, Hsp70 immunoreactivity was
restricted to the outer nuclear layer and inner segments of the retina.
Hsp70 staining was also prominent in basal and wing cells of the limbal
cornea and to a lesser extent in the central corneal epithelium. The
optic nerve was Hsp70 negative.
conclusions. Hsc70 and Hsp70 have distinct distributions in the normal rat eye,
which imply regional and cell-specific
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