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Thomas Wesendahl, Peter Janknecht, Bea Ott, Martin Frenz; Erbium:YAG Laser Ablation of Retinal Tissue under Perfluorodecaline: Determination of Laser–Tissue Interaction in Pig Eyes. Invest. Ophthalmol. Vis. Sci. 2000;41(2):505-512.
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purpose. To evaluate the effect of Er:YAG laser on pig retina using a
perfluorodecaline/retina interphase with the goal of precisely
determining the extent of retinal tissue ablation.
methods. Free running (τ = 250 μsec) Er:YAG laser pulses were
transmitted through a zirconium fluoride (ZrF4) fiber
guarded by quartz rod (d = 1000 μm). Laser pulses
were applied to the retinal surface of enucleated pig eyes. Eyes were
mounted in a specially designed rotating sample holder. The fiber probe
was elevated 1.0 ± 0.3 mm above the retinal surface with
perfluorodecaline serving as transmitting medium. The laser energy was
applied in a circular pattern with a radius of 3.0 mm. Radiant
exposures were set to 1, 3, 5, and 10 J/cm2.
results. Tissue ablation linearly increased with radiant exposure from 3.2 ± 3.7 μm at 1 J/cm2 up to 40.9 ± 12.9 μm at 10
J/cm2. Thermal tissue changes extended 70 ± 10 μm
vertically into the retina and 25 ± 5 μm horizontally.
Distortion of outer photoreceptor segments was noticed when the retina
was exposed to radiant exposures of 3 J/cm2 or higher.
conclusions. The Er:YAG laser in combination with perfluorodecaline produced precise
ablation of the pig retina, which suggests the feasibility of this
technique for safe ablation of epiretinal
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