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Sherry L. Ball, Heywood M. Petry; Noninvasive Assessment of Retinal Function in Rats Using Multifocal Electroretinography. Invest. Ophthalmol. Vis. Sci. 2000;41(2):610-617.
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purpose. To assess the applicability of multifocal electroretinograms (mfERGs)
for evaluation of function in this small-eyed animal with a
rod-dominant retina that is often used to model retinal diseases.
methods. Noninvasive monocular mfERGs were recorded in anesthetized albino
(Sprague–Dawley) and pigmented (Long Evans) rats. Achromatic stimuli
subtending a 49° by 53° field consisted of 61 hexagons that were
generated and presented (at varying rates and luminances) using a
Visual Evoked Response Imaging System (VERIS; EDI, San Mateo, CA). The
VERIS also was used to calculate individual responses and for analysis.
results. mfERGs were recorded from pigmented and albino rats by slowing the rate
of stimulus presentation to allow for the slow recovery time of the rod
system. In each rat strain, responses varied systematically with
changes in stimulus parameters. Peak response amplitude increased as
the rate of stimulation was slowed and as stimulus luminance was
increased. Response latency decreased as stimulus intensity was
increased. The local nature of the response was assessed by several
conclusions. The present work demonstrated the feasibility and limitations of using
mfERG to assess topographical changes in the rat retina. It showed that
despite the problems of the unavoidable self-adapting nature of the
stimulus, the small eye of the animal, and the high potential for light
scatter within the retina, multifocal responses with a good
signal-to-noise ratio can be obtained from the
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