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Jun Sueda, Naofumi Hikita, Manabu Mochizuki, Atsuo Jimi, Masamichi Kojiro; Kinetics of Apoptotic Cells in Experimental Autoimmune Uveoretinitis. Invest. Ophthalmol. Vis. Sci. 2000;41(3):799-804.
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purpose. To investigate the role of apoptosis in immunopathogenic mechanisms of
experimental autoimmune uveoretinitis (EAU), the kinetics of
apoptotic cells and expression of Fas and Fas ligand (FasL) in the eye
with EAU were studied.
methods. Male inbred Lewis rats were immunized with S-antigen (40 μg/rat), and
eyes were examined to detect apoptotic cells on days 1, 4, 8, and 10
post-immunization and days 0, 2, 4, 6, and 8 after the onset of EAU.
The clinical and pathologic scores were used for estimating EAU.
Apoptotic cells were analyzed by TdT-mediated dUTP nick-end labeling,
electron microscopic and immunohistologic examinations, and agarose gel
electrophoresis. The anti–rat Fas and anti–rat FasL antibodies were
used to examine the expression of Fas and FasL.
results. Apoptotic cells were detected in the infiltrating cells in the aqueous
humor, the vitreous body, the iris-ciliary body, and the retina.
Apoptotic cells were observed as early as the day of EAU onset and
reached a peak on day 2 after the disease onset. Fas and FasL were
expressed on the infiltrating cells in the aqueous humor and the
vitreous. FasL was expressed on resident cells of the ciliary body. The
kinetics of the expression of FasL corresponded with the kinetics of
conclusions. Fas–FasL–mediated apoptosis is considered to occur in the eye with
EAU and plays a role in the immunopathogenic mechanisms to eliminate
ocular infiltrating cells, thereby downregulating the inflammatory
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