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Hajib N. Madhavan, K. Lily Therese, Pasricha Gunisha, Udayakumar Jayanthi, Jyotirmay Biswas; Polymerase Chain Reaction for Detection of Mycobacterium tuberculosis in Epiretinal Membrane in Eales’ Disease. Invest. Ophthalmol. Vis. Sci. 2000;41(3):822-825.
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purpose. Tuberculous etiology has been suggested in Eales’ disease. Because
epiretinal membrane (ERM) is formed on the inner surface of the retina
in Eales’ disease, it could be the most appropriate intraocular
specimen for investigation. Therefore, a nested polymerase
chain reaction (nPCR), which detects MPB64 gene of Mycobacterium tuberculosis on the archival specimens of
ERM of well-documented Eales’ and non-Eales’ patients, was applied
and the results compared.
methods. nPCR technique was standardized, and the sensitivity and specificity of
the primers were determined. nPCR technique was applied to tissue
sections obtained from formalin-fixed and paraffin-embedded tissues of
ERM from 23 patients with Eales’ disease and 27 noninfective and
non-Eales’ disease patients as controls.
results. nPCR technique was specific for M. tuberculosis genome
and sensitive enough to detect 0.25 fg (corresponding to the presence
of a single bacillus). Eleven (47.8%) ERM of 23 Eales’ disease and 3
(11.1%) of 27 controls were positive for M.
tuberculosis genome. The difference between the two groups was
statistically significant (P = 0.001), indicating
association of this bacterium with Eales’ disease.
conclusions. The demonstration of the presence of M. tuberculosis DNA
by nPCR technique in significant number of ERM of Eales’ disease
compared with the controls further emphasizes the probable role of this
bacterium in the pathogenesis of this enigmatic clinical
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