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M. Francesca Cordeiro, Shomi S. Bhattacharya, Gregory S. Schultz, Peng T. Khaw; TGF-β1, -β2, and -β3 In Vitro: Biphasic Effects on Tenon’s Fibroblast Contraction, Proliferation, and Migration. Invest. Ophthalmol. Vis. Sci. 2000;41(3):756-763.
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purpose. To compare the effects of the three human transforming growth
factor-β (TGF-β) isoforms and different concentrations of TGF-β
on human Tenon’s capsule fibroblasts (HTF), with a view to delineating
the role of this growth factor in the subconjunctival scarring response
after glaucoma filtration surgery.
methods. Application of recombinant human TGF-β1, -β2, and -β3 (range
0–10−8 M) was assessed using several assays of HTF
function: fibroblast-mediated collagen contraction, proliferation, and
results. All three isoforms of TGF-β behaved in a similar manner in vitro.
They each stimulated HTF-mediated collagen contraction, proliferation,
and migration with a characteristic concentration-dependent response,
with peak activities at 10−9, 10−12, and
10−9 M, respectively, that were significantly different
from control (P < 0.05). At concentrations above
and below peak activities, HTF activity was reduced, demonstrating
biphasic effects of TGF-β.
conclusions. TGF-β1, -β2, and -β3 have similar actions in vitro; this is
demonstrated by their effects on several HTF-mediated functions.
TGF-β induces a response in HTF that is concentration-dependent, with
different functions being maximally stimulated at different
concentrations. This biphasic response highlights the significance of
the concentration profile of TGF-β at the wound site. These findings
are important in filtration surgery, where constant changes in the
local environment occur due to the passage of aqueous and the wound
healing process. The varying levels of TGF-β in the aqueous and
subconjunctival tissues may thus significantly modify the conjunctival
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