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Masayoshi Tachibana, Takashi Kasukabe, Yasuhito Kobayashi, Tomo Suzuki, Shigeru Kinoshita, Yoshibumi Matsushima; Expression of Estrogen Receptor α and β in the Mouse Cornea. Invest. Ophthalmol. Vis. Sci. 2000;41(3):668-670.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. To test the possibility that estrogen has a direct effect on corneal
cells, the possible occurrence of estrogen receptor alpha
(ERα) and beta (ERβ) in the cornea of mice was examined.
methods. To test for the occurrence of ER proteins in the cornea of mice, an
immunocytochemical method was used. To test for the occurrence of ER
mRNAs in the cornea of mice, reverse transcription–polymerase chain
reaction (RT–PCR) was used.
results. Immunocytochemical examination revealed that both ERα and ERβ exist
in the cell nuclei of corneal epithelial, stromal, and endothelial
cells of both male and female mice. RT–PCR revealed that RNAs of ERs
occur in the cornea of both male and female mice.
conclusions. Because ERα and ERβ occur in corneal cells of mice, estrogen may
exert biological functions in corneal cells through direct interaction
with these ERs.
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