Purchase this article with an account.
Carl F. Marfurt, Christopher J. Murphy, John L. Florczak; Morphology and Neurochemistry of Canine Corneal Innervation. Invest. Ophthalmol. Vis. Sci. 2001;42(10):2242-2251. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To determine the architectural pattern and neuropeptide content of
canine corneal innervation.
methods. Corneal nerve fibers in normal dog eyes were labeled
immunohistochemically with antibodies against protein gene product
(PGP)-9.5, calcitonin gene-related peptide (CGRP), substance P (SP),
vasoactive intestinal polypeptide (VIP), and tyrosine hydroxylase (TH).
Relative innervation densities and distribution patterns for each fiber
population were assessed qualitatively by serial line-drawing
reconstructions and quantitatively by computer-assisted analyses.
results. More than 99% of all corneal PGP-9.5–immunoreactive (IR) nerves
contained both CGRP and SP, approximately 30% contained TH, and none
contained VIP. Distribution patterns of corneal PGP-9.5–, CGRP-, SP-,
and TH-IR nerves were indistinguishable, except that TH-IR fibers were
absent from the corneal epithelium. Morphologically, canine corneal
innervation consisted of a rich anterior stromal plexus, divided on the
basis of morphologic criteria into anterior and posterior levels, and a
rich epithelial innervation, characterized by large numbers of
horizontally oriented, basal epithelial “leash” formations. Leash
axons in all quadrants of the corneal epithelium oriented
preferentially toward a common locus in the perilimbal cornea.
conclusions. The results of this study demonstrate for the first time the detailed
architectural features, distinctive basal epithelial leash
orientations, and peptidergic content of canine corneal innervation.
The normal innervation pattern described in this study will provide
other investigators with essential baseline data for assessing corneal
nerve alterations in canine patients with spontaneous chronic corneal
epithelial defects (SCCED) and other ocular diseases or
This PDF is available to Subscribers Only