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C. Albrecht May, Anne V. Fuchs, Michael Scheib, Elke Lütjen-Drecoll; Characterization of Nitrergic Neurons in the Porcine and Human Ciliary Nerves. Invest. Ophthalmol. Vis. Sci. 2002;43(3):581-586.
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purpose. To further characterize a subpopulation of choroidal ganglion
cells associated with the ciliary nerves.
methods. Isolated long ciliary nerves of porcine and human eyes containing
ciliary nerve–associated ganglion cells (CNGCs) were embedded in Epon
for ultrastructural investigation, or wholemounts were stained with
antibodies against nitric oxide synthase (NOS), vasoactive intestinal
polypeptide (VIP), vesicular acetylcholine transporter, neuropeptide Y
(NPY), tyrosine hydroxylase (TH), calcitonin gene-related peptide
(CGRP), substance P (SP), and synaptophysin. In addition, wholemount
preparations of the choroid and of the anterior segment were stained
for reduced nicotinamide adenine dinucleotide phosphate-diaphorase
(NADPH-D). Serial sections through choroid and anterior segment were
stained with the prior antibodies listed.
results. In the porcine choroid only CNGCs were present. They stained for brain
(b)NOS and VIP and were surrounded by SP and VIP-immunoreactive
(IR) nerve terminals. The axonal processes of the CNGCs followed the
ciliary nerves to the anterior eye segment, where they formed a nerve
fiber plexus that terminated in the trabecular meshwork. None of the
axons passed into the sparse NOS-IR nerve fiber plexus surrounding the
choroidal vasculature. The CNGCs in the human choroid morphologically
resembled those seen in the pig.
conclusions. The CNGC proportion of choroidal ganglion cells is presumably involved
in the intrinsic (peripheral) innervation of the aqueous outflow
tissues and of the choroid.
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