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Stephen J. Kim, Meifen Zhang, Barbara P. Vistica, Chi-Chao Chan, De-Fen Shen, Eric F. Wawrousek, Igal Gery; Induction of Ocular Inflammation by T-Helper Lymphocytes Type 2. Invest. Ophthalmol. Vis. Sci. 2002;43(3):758-765.
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purpose. Sight-damaging ocular inflammation is often mediated by
T-helper (Th) lymphocytes. The population of Th cells is divided into
two major subsets, designated Th1 and Th2, that differ by their
cytokine production and biological activities. In the present study,
the capacity of Th1 and Th2 cells to induce ocular inflammation was
methods. Ocular inflammation was induced in transgenic (Tg) mice that express
hen egg lysozyme (HEL) in their lens, by adoptively transferring Th
cells that transgenically express HEL-specific receptor. Th1 and Th2
populations were polarized in vitro, and their selective cytokine
production was determined by conventional methods. Levels of ocular
inflammation were monitored by conventional histologic methods.
Infiltrating cells were collected from sections of inflamed eyes by
microdissection, and their cytokine production was examined by RT-PCR.
results. Th1 cells were highly immunopathogenic, producing disease in naive
recipients at numbers as low as 0.12 × 106, whereas
Th2 cells were inactive in these recipients, even at 30 ×
106. Th2 cells, however, produced inflammation when
transferred into sublethally irradiated recipients. Distinctive
histopathologic changes characterized ocular inflammation induced by
the two types of Th cells. Cytokine analysis of infiltrating cells in
recipient mouse eyes, as well as of splenocytes of these mice
demonstrated that the transferred cells retained their type
specificity. Coinjecting Th2 and Th1 cells did not alleviate the ocular
disease in naive recipients and even exacerbated the immunopathogenic
process in irradiated recipients.
conclusions. Th2 cells are capable of inducing ocular inflammation, but only in
immunodeficient mice, and are profoundly inferior to Th1 cells in their
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