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Judite Scher, Ellen Wankiewicz, Gregory M. Brown, Hiroki Fujieda; MT1 Melatonin Receptor in the Human Retina: Expression and Localization. Invest. Ophthalmol. Vis. Sci. 2002;43(3):889-897.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. Melatonin’s function in human vision is far from understood, in part
because of the lack of information on its cellular targets. Therefore,
expression and localization of the MT1 melatonin receptor
in human retina was examined.
methods. Postmortem nonpathologic human eyes from nine donors were investigated,
three by reverse transcription–polymerase chain reaction (RT-PCR) for
MT1 and MT2 transcripts and six by
immunocytochemistry, using a peptide-specific anti-MT1 receptor antibody.
results. RT-PCR suggested that both MT1 and MT2 transcripts had similar levels of expression. Vertical slices of human
retina demonstrated MT1 immunoreactivity in cell bodies
along the outer border of the inner nuclear layer (INL), along the
inner border of the INL, in cell bodies within the ganglion cell layer
(GCL), and in the inner segments (IS) of photoreceptors. Double
immunolabeling using anti-MT1 and tyrosine hydroxylase
revealed that 69% of CA1 and 63% of CA2 dopaminergic neurons
exhibited MT1 immunoreaction. Double immunolabeling with
anti-parvalbumin, a horizontal cell marker, showed that
MT1-positive cells along the outer INL border were
exclusively horizontal cells, and that 18% of horizontal cells in
central retina expressed MT1. Double staining with
MT1 and markers for both rod and cone photoreceptors
suggest that IS staining is present on rod cells.
conclusions. The MT1 receptor is expressed in diverse neuronal cell
types in the human retina, providing evidence of a significant role for
melatonin and this receptor subtype in human
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