June 1970
Volume 9, Issue 6
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Articles  |   June 1970
Holographic Recording of a Retina Using a Continuous Wave Laser
Author Affiliations
  • JOSEPH L. CALKINS
    Department of Ophthalmology, The University of Michigan Medical Center, and the Radar and Optics Laboratory, Institute of Science and Technology, The University of Michigan, Ann Arbor, Mich.
  • CARL D. LEONARD
    Department of Ophthalmology, The University of Michigan Medical Center, and the Radar and Optics Laboratory, Institute of Science and Technology, The University of Michigan, Ann Arbor, Mich.
Investigative Ophthalmology & Visual Science June 1970, Vol.9, 458-462. doi:
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    • Get Citation

      JOSEPH L. CALKINS, CARL D. LEONARD; Holographic Recording of a Retina Using a Continuous Wave Laser. Invest. Ophthalmol. Vis. Sci. 1970;9(6):458-462.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

A new method for examining and recording features of the eye has been developed. Using holography rather than photography, we obtained, in a single exposure of a cat's eye, a three-dimensional image containing information about the retina as well as all layers along the optical path. Recording a hologram of a living object posed special problems of physical stability necessitating short exposure intervals. With shorter exposure intervals one needs higher levels of retinal illumination to adequately expose the photographic emulsion, and, unless care is exercised, these high levels of light energy may damage the retina. To optimize conditions for the exposure, the emulsion sensitivity was increased and the anesthesized cat was kept motionless. Agfa 10E70 emulsion was employed and special processing used to increase its speed. The light source was a continuous-wave helium-neon laser with an output of 58 mw. Light energy incident on the retina was only 1,200 erg/cm.2, whereas Department of Health, Education, and Welfare (HEW) guidelines place safe exposures between 50,000 and 100,000 erg/cm2.

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