June 1970
Volume 9, Issue 6
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Articles  |   June 1970
Experimental Band Keratopathy Electron Microprobe X-Ray Analysis of Aqueous and Corneal Calcium Concentrations
Author Affiliations
  • DONALD J. DOUGHMAN
    Departments of Ophthalmology and Physiology, College of Medicine, University of Iowa, Iowa City, Iowa.
  • MARY JO INGRAM
    Departments of Ophthalmology and Physiology, College of Medicine, University of Iowa, Iowa City, Iowa.
  • WILLIAM M. BOURNE
    Departments of Ophthalmology and Physiology, College of Medicine, University of Iowa, Iowa City, Iowa.
Investigative Ophthalmology & Visual Science June 1970, Vol.9, 471-475. doi:
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      DONALD J. DOUGHMAN, MARY JO INGRAM, WILLIAM M. BOURNE; Experimental Band Keratopathy Electron Microprobe X-Ray Analysis of Aqueous and Corneal Calcium Concentrations. Invest. Ophthalmol. Vis. Sci. 1970;9(6):471-475.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Quantitative electron microprobe x-ray analyzer (EMX) assays of calcium concentrations in the aqueous and cornea of rabbits under various conditions, including experimental band keratopathy, are presented. Aqueous calcium values were significantly lowered in band keratopathy. The corneal calcium concentration remained constant in all the conditions tested throughout all layers of the cornea, with the exception of band keratopathy where an abrupt 25- to 100-fold increase occurred in the sub epithelial layers. These findings are discussed, as well as our evaluation of the EMX in ophthalmology research.

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